Table 1. Differentially abundant proteins in hermaphroditic and male gametophytes from C. thalictroides.
| Spot No.a | Protein description | Reference species | Accession No.b | Exp. MW/pIc | Thr. MW/pId | #e | SCf | Ratiog |
|---|---|---|---|---|---|---|---|---|
| Protein folding and refolding (11) | ||||||||
| 2251 | Chaperonin 60 subunit beta 2 |
Ricinus communis | XP_002523404.1 | 59354/5.42 | 64490/5.65 | 11 | 163 | 2.25±0.17 |
| 2163 | Chaperonin 60–2 | Physcomitrella patens | XP_024366438.1 | 62062/5.67 | 61757/5.88 | 4 | 78 | - |
| 7814 | Heat shock protein 70 | P. patens | XP_024361882.1 | 73140/5.44 | 73140/5.94 | 9 | 123 | 1.47±0.22 |
| 7815 | Heat shock protein 70 | P. patens | XP_024361882.1 | 64306/5.39 | 73140/5.94 | 7 | 183 | 1.68±0.07 |
| 7827 | Heat shock protein 70 | Oryza sativa | ACJ54890.1 | 76511/5.20 | 71945/5.30 | 17 | 423 | 1.58±0.42 |
| 7829 | Heat shock protein 90–1 | Nicotiana benthamiana | AAR12193.1 | 80339/5.18 | 80339/4.94 | 22 | 431 | 0.62±0.11 |
| 7857 | Peptidyl-prolyl cis-trans isomerase | Spinacia oleracea | XP_021866579.1 | 50832/4.84 | 50068/5.29 | 5 | 106 | 2.01±0.38 |
| 7852 | Rubisco large subunit-binding protein subunit alpha | R. communis | XP_002534347.2 | 62164/4.95 | 53280/5.25 | 9 | 94 | 1.45±0.13 |
| 7853 | Rubisco large subunit-binding protein subunit alpha | Sorghum bicolor | XP_002440887.1 | 60913/5.03 | 60913/5.07 | 9 | 121 | 1.77±0.15 |
| 2227 | Rubisco large subunit-binding protein subunit beta | Zea mays | NP_001306697.1 | 59583/5.89 | 61968/5.42 | 10 | 289 | 1.59±0.21 |
| 2317 | T-complex protein 1 subunit beta | Vitis vinifera | XP_002285912.1 | 58957/6.08 | 57628/5.60 | 11 | 112 | 0.63±0.07 |
| Photosynthesis and chloroplast (8) | ||||||||
| 1342 | ATP-dependent Clp protease | Vitis vinifera | XP_010663794.1 | 102347/6.32 | 99169/6.09 | 19 | 239 | 1.63±0.26 |
| 7424 | Lactoylglutathione lyase | P. patens | XP_024375349.1 | 34416/5.49 | 29627/5.23 | 5 | 168 | 1.99±0.09 |
| 3181 | LL-diaminopimelate aminotransferase | P. patens | XP_024392750.1 | 53048/6.08 | 50746/8.00 | 3 | 113 | 1.69±0.14 |
| 7869 | Magnesium-chelatase subunit ChlI | V. vinifera | RVW29648.1 | 49864/5.39 | 46692/5.68 | 11 | 108 | 2.13±0.21 |
| 7344 | Oxygen-evolving enhancer protein 1 | Populus trichocarpa | XP_002307234.1 | 35348/5.01 | 35348/5.89 | 6 | 402 | 1.70±0.09 |
| 7832 | Ribulose bisphosphate carboxylase/oxygenase activase | Populus trichocarpa | XP_002312110.3 | 83226/4.94 | 40277/5.36 | 10 | 386 | 1.92±0.03 |
| 2281 | Ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit | Palhinhaea pendulina | CAC22277.1 | 59241/6.15 | 49006/6.49 | 12 | 73 | 4.28±0.85 |
| 2449 | Ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit | Dryopteris sublacera | ABF59846.1 | 58170/6.08 | 49148/6.54 | 14 | 91 | 0.63±0.01 |
| Metabolism (8) | ||||||||
| 7811 | 2,3-bisphosphoglycerate-independent phosphoglycerate mutase | Mesembryanthemum crystallinum | Q42908.1 | 60476/5.61 | 61316/5.39 | 4 | 85 | 1.53±0.16 |
| 3035 | 3-ketoacyl-CoA thiolase 2 | Prosopis alba | XP_028773475.1 | 54155/6.30 | 48962/6.36 | 8 | 168 | 0.42±0.02 |
| 7510 | Arginase 1 | P. patens | XP_024376768.1 | 42118/5.86 | 37423/6.06 | 2 | 76 | 0.64±0.05 |
| 7451 | Cysteine synthase-like | P. patens | XP_024360091.1 | 38585/5.77 | 41650 /8.21 | 6 | 223 | 1.62±0.43 |
| 3389 | Glutamine synthetase | Cryptomeria japonica | BBA84049.1 | 50238/6.20 | 39732/5.96 | 3 | 208 | 0.53±0.10 |
| 7531 | Glutathione S-transferase | V. vinifera | XP_002263395.1 | 27676/6.47 | 25740/6.06 | 2 | 92 | 2.07±0.29 |
| 7487 | Glutathione S-transferase DHAR1 | P. patens | XP_024357492.1 | 74303/5.96 | 25502/7.99 | 4 | 92 | 0.53±0.01 |
| 8037 | Malate dehydrogenase | Beta vulgaris | NP_001290006.1 | 45437/5.90 | 35810/5.89 | 7 | 97 | 0.55±0.06 |
| Protein synthesis (2) | ||||||||
| 3194 | Elongation factor tub | N. sylvestris | XP_009772722.1 | 52769/6.15 | 52769/5.95 | 6 | 152 | 1.72±0.07 |
| 7245 | Translation initiation factor 5A | Dendrocalamus sinicus | ABW78939.1 | 15509/5.89 | 17752/7.08 | 5 | 187 | 0.63±0.02 |
| Cell structure (4) | ||||||||
| 2861 | Actin 2 | Anemia phyllitidis | AAC64127.1 | 53023/5.33 | 41827/5.31 | 15 | 340 | + |
| 6415 | Actin | P. patens | XP_024374057.1 | 36460/5.25 | 41810/5.3 | 6 | 76 | + |
| 1816 | Actin | Mesostigma viride | O65316.1 | 69752/5.97 | 41790/5.3 | 6 | 84 | + |
| 7199 | Centrin | Pterosperma cristatum | CAA58719.1 | 17020/4.94 | 15320/4.38 | 3 | 179 | 0.25±0.06 |
a: the numbering corresponds to the matched IDs in the 2D gels
b: the database accession number from the NCBI protein database
c, d: the experimental and theoretical molecular weight (Da) and pI of the identified proteins
e: number of peptides identified by MS/MS
f: mascot score resulting from the LC-MS/MS search
g: the relative fold change in abundance levels when compared with the abundance in male gametophytes (p ≤ 0.01); (+) denotes a unique spot in hermaphroditic gametophytes, (-) denotes a unique spot in male gametophytes. The average and standard deviation are presented.