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. 2019 Aug 19;14(8):e0221104. doi: 10.1371/journal.pone.0221104

Fig 8. Malat1 functions as CeRNA to regulate Lamp1 expression by sponging mir-23-3p in macrophage cells.

Fig 8

(A) RT-qPCR was performed to evaluate the expression levels of Malat1, mir-23b-3p, and Lamp1 in Raw264.7 cells respective treatment with 50 nM Rapa 2h or 3 mM 3-MA 12h (Mean±SD; n = 3). (B) RT-qPCR was used to test mir-23b-3p expression in the different groups, including NC, Si-NC, Malat1-siRNA, Malat1-siRNA+inhibitor-NC (INC), Malat1-siRNA+inhibitor, or inhibitor (Mean±SD; n = 3). (C) The bioinformatics predicted MRE of mir-23b-3p on Malat1. (D) Luciferase reporter assay was performed in 293T cells co-transfected with pmirGLO-Malat1-Wt or Mut and mimics or mimics-NC (Mean±SD; n = 3). (E) Luciferase reporter rescue experiment was conducted to confirm co-transfected pmirGLO-Malat1-Wt, pmirGLO-Lamp1-Wt and mir-23b-3p mimics effect the relative luciferase activity in 293T cells (Mean±SD; n = 3). (F) WES was used to confirm over-expression Malat1 can promote Lamp1 expression in Raw264.7 cells. (G) Graphic summary of Malat1 function in this work.