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Si-H carbene insertion activity of Mb(H64V,V68A) and proximal ligand variants thereof.a
 | ||||
|---|---|---|---|---|
| Entry | Protein | Proximal Ligand |
Yieldb | TON |
| 1 | Mb(H64V,V68A) | His | 69% | 345 |
| 2 | Mb(H64V,V68A,H93D) | Asp | 66% | 330 |
| 3 | Mb(H64V,V68A,H93F) | Phe | 50% | 250 |
Reaction conditions: 10 mM 1a, 10 mM EDA, 20 μM Mb variant in 50 mM phosphate buffer (pH 7), 1 hour, r.t., in anaerobic chamber.
Based on GC conversion using calibration curves with isolated 3a.
