Figure 3. Taz1 associates with Taz1-dependent heterochromatin islands.
(A) Distribution of H3K9me2 and Taz1-GFP along S. pombe chromosomes as determined by ChIP-chip. Islands affected in mut-5 cells are labeled in red. H3K9me2 data from (Zofall et al., 2012) was used. (B, C) Taz1 preferentially localizes to non-DSR islands containing telomeric repeat sequences. Note that island 7 contains a putative variant binding sequence. (B) Taz1-GFP distribution at non-DSR islands as determined by ChIP-chip. ORFs, the positions of Taz1 binding sites and late replicating origins are indicated. (C) Conventional ChIP of Taz1-GFP at the indicated islands. The average enrichments and standard deviations of three measurements are indicated. (D) Taz1-GFP distribution at DSR islands is plotted. See also Figure S3.