Table 1.
List of proteins identified by pulldown assay with the C-terminal region of YbdG
| Mass | Amino acid sequence | Protein | Gene | |
|---|---|---|---|---|
| kDa | ||||
| 1 | IVQ | MPILGYWKIKGLVQPSW | GST | Fusionsa |
| 2 | 34.1 | MIIVTGGAGFIGSNI | ADP-l-glycero-d-manno-heptose-6-epimerase | hldD |
| 3 | 21.7 | MKVADLVVSLAYYVQb | FKBP-type peptidyl-prolyl cis-trans-isomerase | slyD |
| 4 | AEITAVL | NDc | ||
| 5 | 39.6 | SKIFDFVKPGVITGD | Fructose-bisphosphate aldolase class 2 | fbaA |
| 6 | APVDNTK | ND | ||
| 7 | MPLFYKPGAVSLASP | GST | gstAd | |
| 8 | MLG | ND | ||
| 9 | XXXXFLVM | ND | ||
| 10 | XXXXLXDVIAEFA | ND |
a Pseudomonas GST in bait fusion proteins.
b One lysine residue is missing in the sequence of SlyD: MKVAKDLVVSLAYYVQ.
c ND, not determined.
d E. coli endogenous GST (number 7) was isolated due to its affinity to glutathione beads.