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Alkaline phosphatase activity in CDF family mutants under steady-state growth conditions. Alkaline phosphatase (ALP) activity was assayed in the indicated strains, which were grown in ZL-EMM supplemented with 0, 1, 10, or 100 μm zinc to an A600 of ∼5. Activity is the mean of three independent repeats, with the error bars representing standard deviations. **, p < 0.01; *, p < 0.05; ND, not determined.
