Figure 8.

Zhf1 and the Cis4–Zrg17 complex have a redundant role in the activation of Pho8. A, the indicated strains were grown overnight in ZL-EMM to an A₆₀₀ of ∼5 (t = −0). Cells were then exposed to 100 μm zinc and harvested for alkaline phosphatase (ALP) activity assays at the indicated time points. Activity is the mean of three independent repeats, with the error bars representing standard deviations. B, the indicated strains expressing Pho8-HA or the empty vector were subject to zinc shock as described above, and alkaline phosphatase activity was assayed at the indicated time points. Activity is the mean of three independent repeats, with the error bars representing standard deviations.