Figure 4.

Effect of Cpt1b deficiency on the TCA cycle. GSEA identified up-regulation (red) of genes involved in the TCA cycle in Cpt1b^M−/− (KO) mice (A). Metabolomics screening identified a high number of metabolites linked to carbon entry into the TCA cycle (B), TCA cycle intermediates (D), and reducing equivalents (E). GSEA revealed enrichment of the coenzyme A (CoA) biosynthesis pathway. Compiled results from the SAGE and metabolomics platforms are depicted in F. *, p < 0.05. The abbreviations used are as follows: α-KG, α-ketoglutarate; Succ-CoA, succinyl-CoA.