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. 2019 Jun 27;294(33):12483–12494. doi: 10.1074/jbc.RA119.008666

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© 2019 Duval et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 4. — kinetic analysis of PTPN2 inhibition by BQ. A, recombinant PTPN2 was incubated with increasing concentrations of BQ. Aliquots were taken every 30 s, and residual PTPN2 activity was measured using a pNPP assay. For each BQ concentration, the natural logarithm (ln) of the percentage of residual PTPN2 activity was plotted as a function of time. The apparent first-order inhibition constant (k_obs) was calculated from the linear regression (slope). B, k_obs values were plotted against BQ concentrations, and the second-order inhibition constant (k_inact) was extracted from the slope. C, ln(k_obs) were plotted as a function of ln[BQ], and the stoichiometry of the reaction was extracted from the slope.