Fig 1:

Aprotinin acts synergistically with GM6001 to inhibit vasculogenesis in endothelial cell (EC) co-cultures with dermal fibroblasts (DFs) and lung fibroblasts (LFs) but not in co-cultures with bone-marrow mesenchymal stem cells (MSCs) in 2.5 mg/mL fibrin hydrogels. Representative images of capillary-like networks formed in EC-DF co-cultures after 7 d with indicated inhibitor concentrations are shown stained with the endothelial-selective lectin from Ulex europaeus (UEA, red) to highlight networks (A). Scale bar = 500 μm. Multiple images at prespecified locations were acquired for each condition for co-cultures of ECs and DFs (B), LFs (C), or MSCs (D) for 3 independent experiments and network lengths were quantified per volume as outlined in the methods. Two-way ANOVA (GM6001, aprotinin, interaction) results: EC-DF (p < 0.0001, p = 0.0218, p = 0.0349), EC-LF (p < 0.0001, p = 0.0059, p = 0.0009), EC-MSC (p < 0.0001, p = 0.0177, p = 0.459).