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. 2019 Aug 19;9:11966. doi: 10.1038/s41598-019-48333-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PGR mRNA and protein are induced by the LH surge in granulosa cells. (A) PGR mRNA expression in eCG and hCG-primed granulosa cells at no eCG + hCG (unstim) and eCG + hCG 0–12 h post-hCG stimulation. RT-qPCR was performed on samples of 1–3 ovaries. N = 3 independent experiments, bars with different superscripts are significantly different, F_6,14 = 17.53, p < 0.0001 (one-way ANOVA). (B) Western blot of PGR in granulosa cells during ovulation (unstim or eCG + hCG 0–12 h post-hCG). Western blot was performed in biological triplicates, with 3–4 mice per time point in each replicate. One representative example of three highly similar results is shown. Full-length blot is available in Suppl. Data 3.