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. 2019 Aug 19;9:11966. doi: 10.1038/s41598-019-48333-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PGR-dependent differentially expressed genes in PRKO vs PGR +/− peri-ovulatory granulosa cells. (A) Principal components analysis (PCA) plot of granulosa cell microarray samples. (B) Volcano plot of granulosa cell microarray data. A total of 367 genes with log fold change >1 or <−1 are plotted. The horizontal dashed line indicates p-value cut-off (p-value < 0.01, or –log(p-value) >2). The vertical dashed lines indicate fold change cut-off (logFC <−1 or >1). Genes that meet these criteria are indicated as blue symbols. Genes that have been selected for panel C are indicated as red symbols. (C) Examples of genes that are significantly differentially expressed in PRKO granulosa cells. DEG selected are genes that were differentially expressed (FC >2 or <−2) with a p-value cut-off of 0.01. These genes are annotated as red points in panel B. (D) Canonical pathway analysis of PGR-regulated DEG identified in microarray. 61 DEG that were determined from granulosa cell microarray were analysed for enriched pathways using the IPA software. Pathways with a -log(p-value) cut-off above 2 (or p-value < 0.01) were determined to be significantly enriched. (E) IPA upstream regulator analysis of genes identified in microarray, showing upstream regulators that were significantly enriched (p-value < 0.01).