Table 3.
Summary of the methyltransferases from A. woodii
| Gene name (DSMZ 1030) | Gene product | Predicted specificity | Motif detected | Mean mod. QV | Coverage |
|---|---|---|---|---|---|
| Type I | |||||
|
Awo_c04420 Awo_c04450 |
R-M subunit HsdM1 R-M subunit HsdS1 |
N.A. | N.D. | N.D. | 2075 |
|
Awo_c08800 Awo_c08810 |
R-M subunit HsdM2 R-M subunit HsdS2 |
TAAGNNNNNTCC |
TAA(m6A)GNNNNNTCC GGA(m6A)NNNNNCNTA |
1914 1567 |
3446 |
|
Awo_c17130 Awo_c17140 |
R-M subunit HsdS3 R-M subunit HsdM3 |
GATGNNNNNNTGC |
GA(m6A)TNNNNNNNTGC GCA(m6A)NNNNNNCAT |
1136 932 |
1768 |
| Awo_c17840 | R-M subunit HsdM4 | N.A. | N.D. | N.D. | 8535 |
| Type II | |||||
| Awo_c06460 | DNA (cytosine-5-)-methyltransferase Dcm | CCGG | N.D. | N.D. | 8569 |
| Awo_c14460 | DNA modification methyltransferase | N.A. | CAAAAA(m6A)R | 2185 | 6372 |
| Awo_c18570 | Putative DNA methylase | N.A. | N.D. | N.D. | 9517 |
| Awo_c18590 | Putative DNA methylase | CCWGG |
TNNNCCTG(U.K)*GNGNNV MNNBSNTNNCCNG(U.K)*G |
48 32 |
8514 |
| Awo_c19740 | Potential Type II | GCCRAG | GCCRAG | 1986 | 6223 |
| Awo_c30860 | Putative DNA methylase | N.A. |
TA(m6A)GNHNNNNV BCANA(m6A)NNGNNNNNM VNTNNNNNNTTNTA(m6A) |
608 172 147 |
5539 |
| Awo_c31130 | DNA (Cytosine-5-)-methyltransferase YdiP | N.A. | N.D. | N.D. | 11339 |
| Awo_c34920 | N6 adenine-specific DNA methylase | N.A. | WBNNMDVA(m6A)GCNH | 70 | 937 |
| Awo_c35180 | phage N6 methylase | TGGCCA | WNNNNNMGNC(m4C)NGGNM | 237 | 708 |
| Awo_c35200 | Lambda C methyl | N.A. | N.D. | N.D. | 909 |
| Neg-control | bgaB (beta-galatosidase) | None | N.D. | N.D. | 8051 |
Methyltransferases from A. woodii cloned individually and their corresponding motifs
*indicates the modification position is misleading, as the modification on a guanine is not likely, in addition m5C has typical characteristic kinetic signals two and six bases downstream of the methylated position
N. A. not available, N. D. not detected, U. K unknown