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. 2019 Aug 19;10(9):629. doi: 10.1038/s41419-019-1845-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a The miR-342-3p expression levels in normal brain tissues (NBTs), and glioma tissues of different grades. Data are presented as the mean ± SD (n = 10, each group). ^ΔΔP < 0.01 vs. NBTs group; **P < 0.01 vs. Grade I–II group; ^#P < 0.05 vs. Grade III group. b The expression of miR-342-3p in human astrocytes (HA) and glioblastoma cell lines (U87 and U251). c The expression of miR-342-3p after Linc-00313 knockdown in U87 and U251 cells. d The predicted miR-342-3p binding site in the Linc-00313 sequence (Linc-00313-Wt) and the designed mutant sequence of miR-342-3p binding site (Linc-00313-Mut) are indicated. Relative luciferase activity was conducted after cells were transfected with Linc-00313-Wt or Linc-00313-Mut. Data were presented as the mean ± SD (n = 3, each group). **P < 0.01 vs. Linc-00313Wt + Agomir-342-3p-NC. e Cell Counting Kit-8(CCK-8) assay was used to measure the effect of miR-342-3p on the proliferation of U87 and U251. f The apoptotic percentages of U87 and U251 cells were detected after miR-342-3p overexpression or inhibition. g Transwell assays was used to measure the effect of miR-342-3p on the migration and invasion of U87 and U251 cells. Scale bars represent 40 μm. Data are presented as the mean ± SD (n = 3, each group). *P < 0.05 vs. Agomir-342-3p-NC group; ^##P < 0.01 vs. Antagomir-342-3p-NC group. h The miR-485-5p expression levels in normal brain tissues (NBTs), and glioma tissues of different grades. Data are presented as the mean ± SD (n = 10, each group). ^ΔΔP < 0.01 vs. NBTs group; **P < 0.01 vs. Grade I–II group; ^#P < 0.05 vs^. Grade III group. i The expression of miR-485-5p in human astrocytes (HA) and glioblastoma cell lines (U87 and U251). j The expression of miR-485-5p after Linc-00313 knockdown in U87 and U251 cells. k The predicted miR-485-5p binding site in the Linc-00313 sequence(Linc-00313-Wt) and the designed mutant sequence of miR-485-5p binding site (Linc-00313-Mut) are indicated. Relative luciferase activity was conducted after cells were transfected with Linc-00313-Wt or Linc-00313-Mut. Data were presented as the mean ± SD (n = 3, each group). **P < 0.01 vs. Linc-00313Wt + Agomir-485-5p-NC. l Cell Counting Kit-8 (CCK-8) assay was used to measure the effect of miR-485-5p on the proliferation of U87 and U251. m The apoptotic percentages of U87 and U251 cells were detected after miR-485-5p overexpression or inhibition. n Transwell assays was used to measure the effect of miR-485-5p on the migration and invasion of U87 and U251 cells. Scale bars represent 40 μm. Data are presented as the mean ± SD (n = 3, each group). *P < 0.05 vs. Agomir-485-5p-NC group; ^##P < 0.01 vs. Antagomir-485-5p-NC group