Fig. 3. UPF1 binds Linc-00313 and enhances the effect of Linc-00313 on glioma.

a Western blot assay were used to detect the Zic4 expression regulated by UPF1 and Linc-00313. b CCK-8 assay was used to measure the proliferation of U87 and U251 regulated by UPF1 and Linc-00313. c RNA IP assay was used to detect the binding between UPF1 and Linc-00313. d Linc-00313 RNA half-life measured by qRT-PCR after actinomycin D treatment. e Flow cytometry analysis to evaluate the effect of UPF1 and Linc-00313 on the apoptosis of U87 and U251 cells. f Transwell assays was used to measure the effect of UPF1 and Linc-00313 on the migration and invasion of U87 and U251 cells. Data are presented as the mean ± SD (n = 3, each group). *P < 0.05 vs. UPF1(−)NC + sh-NC