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. 2019 Aug 19;9:12040. doi: 10.1038/s41598-019-48475-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analyses of three-dimensional features of isolated AuNPs by stereo TEM reveal true shapes of the particles. Images of triangle (A), pentagon (D) and hexagon (G) captured at +5° have different shading patterns in their respective images captured at −5° (B,E,H) identifying them as tetrahedron (C), pentagon (F) and pentagonal prism (I). Images of two-sided pentagonal pyramids at +45° (J,M), 0° (K,N) and −45° (L,O) illustrate how the particles can be identified as rhomboids in regular TEM. Arrows indicate the particles under observation.