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. 2019 Aug 19;9:12064. doi: 10.1038/s41598-019-48590-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Morphological difference of NPC43 cells cultured with or without the Rock inhibitor (RI) Y27632 in 2D and 3D culture conditions. (B) Percentage of number of viable NPC43 cells/colonies in 2D/3D culture in the absence of RI compared with in the presence of RI (4 μM). Numbers of viable cells were determined by MTT assay on day 3 after the treatment. (C) Comparison of number of viable cells of NPC43 cells treated with or without RI (4 μM) in response to PS1145 (32 μM). Numbers of viable cells were determined by MTT assay on day 3.