Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 19;9:12064. doi: 10.1038/s41598-019-48590-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

(A) Apoptosis analysis of the effects of PS1145 in NPC43 cells in the presence or absence of the Rock inhibitor (RI) Y27632. The effects of 4 μM RI in response to 32 μM PS1145 on the formation of apoptotic cells were detected by TUNEL assay on day 3 after the treatment. The bar chart shows the percentage of number of apoptotic cells formed by the treatment with PS1145 in the presence or absence of Y27632. A parallel MTT assay was performed for the same batch of cell lines. (B) Gene expression analysis of stemness and differentiation genes in NPC43 cells cultured with or without 4 μM Rock inhibitor (RI) Y27632. (C) Western blot analysis of the protein expression of phospho-p65 (pp65) and KLF4 after removal or addition of the RI (4 μM) in NPC43 cells is shown. The treatment time is indicated. Both long and short exposure versions for detection of pp65 are shown in Fig. S6, and only the long exposure is shown in this figure. The differences of pp65 expression at 8 hours is more obvious in the short exposure version.