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. 2019 Aug 19;9:12064. doi: 10.1038/s41598-019-48590-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Comparison of cell viability of the RI-dependent versus the RI-independent NPC43 cells in response to PS1145 (32 μM). The cell viability was determined by MTT assay on day 3 after the treatment. *p value < 0.05. (B) Western blot analysis of the protein expression of KLF4 after siRNA knockdown in NPC43 cells. siCTL, scramble control siRNA. (C) CFA analysis of the effects of KLF4 siRNA on the colony-forming abilities of NPC43 cells. These experiments were conducted in triplicates. *p value < 0.05. (D) Cell viability in response to PS1145 (16 μM) after treatment with KLF4 siRNA in NPC43 cells (in the absence of RI). MTT assay was performed to detect the cell viability. *p value < 0.05.