Fig. 4.

Adeno-associated virus-inner ear (AAV-ie) has a minimal adverse effect on auditory in injected mice. a Representative epi-fluorescence image of P30 AAV-ie-NLS-mNeonGreen (nuclear localization sequence-mNeonGreen)-injected cochlea. The mice were injected at P3 at a dose of 1 × 10¹⁰ genome-containing (particles) (GCs) per ear. Scale bar, 100 µm. b Scanning electron microscopy (SEM) images of P30 control and AAV-ie-NLS-mNeonGreen-injected cochlea at apical, middle, and basal regions. Scale bar, 5 µm. c Magnification SEM images of outer hair cell (OHCs) and inner hair cells (IHCs) of P30 control and AAV-ie-NLS-mNeonGreen-injected cochlea at apical, middle, and basal regions. Scale bar, 1 µm. d OHCs and IHCs numbers per 100 µm of P30 control and AAV-ie-NLS-mNeonGreen-injected cochlea. N = 3 mice. p Value is calculated by Student’s t test. n.s. refers to no significance. Source data are provided as a Source Data file. e Auditory brain-stem response (ABR) thresholds from AAV-ie-NLS-mNeonGreen-injected and -uninjected ears on day 26 after surgery at P3. Error bars ± SEM. N = 4 mice, respectively. Source data are provided as a Source Data file