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. 2019 Aug 19;9:12017. doi: 10.1038/s41598-019-48453-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The bar chart represents the cumulative coefficient of variations (CV) of ten different biochemical classes using six different extraction solvents. (a) CVs of the fecal metabolome from adult giant panda. (b) CVs of the fecal metabolome from baby giant panda. The major metabolite classifications are alkanes, amino acids, amino acid derivatives, benzoic acids and derivatives, long-chain saturated fatty acids, long-chain unsaturated fatty acids, medium-chain saturated fatty acids, nicotinamides and vitamins, and secondary metabolites, and TCA cycle intermediates. The x-axis is the total CV for all major metabolite classes.