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. 2019 Aug 19;9:12059. doi: 10.1038/s41598-019-48557-8

Figure 1.

Figure 1

Epithelial tubular cells go into proliferative cycle upon obstruction of kidney. (a) Staining of Hematoxylin and Eosin (HE) of kidney slices obtained from various time points after obstruction. Sham-operated kidneys were used as a control. Images were taken at x100 magnification. (b) Masson’s trichrome staining. Fibrosis stained in blue. Images were taken at x400 magnification. (c) Co-immunostaining with Anti-Ki67 (green) and Anti-E-Cadherin (red). Ki67 was used for the identification of proliferative cells and E-Cadherin stained epithelial tubular cells. Bar, 100 µm. (d and e) Quantification of the number of Ki67 positive cells (d, n = 15) and E-Cadherin stained area (e, n = 15). (f) The number of Ki67 positive cells on E-Cadherin stained area (the number of Ki67 and E-Cadherin double stained area, n = 15). Data are as given averages ± SD (Standard Deviation); ***P < 0.001 (two-tailed unpaired Student’s t-test).