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. 2019 Aug 19;9:12059. doi: 10.1038/s41598-019-48557-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Activation of the DNA damage checkpoint and Wnt/β-catenin are induced in late stage of renal damage. (a) Immunoblotting with indicated antibodies of tissue lysates from indicated samples. (b) Co-immunostaining with Anti-p-H2A.X^S139 (γH2A.X) (green) and E-Cadherin (red). Scale bar, 100 µm. (c) Immunoblotting of tissue lysates from indicated samples. Of note, γH2A.X was clearly detected 7 days after the injury in our hands. (d) Immunoblotting with indicated antibodies of tissue lysates from indicated samples. The molecular weights (kDa) are shown in the right side of the images. Arrows indicate the height of intended bands. Non-phosphorylated β-catenin means active form of β-catenin.