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. 2019 Aug 19;9:12009. doi: 10.1038/s41598-019-48519-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Lateral LMC axon growth responses to netrin-1 are sensitised by ephrin-A5. Axon outgrowth preference on protein stripes exhibited by lateral LMC axons. left panels: explanted lateral (EphA4+) LMC neurites on netrin-1 (N)/Fc (a,b) or ephrin-A5 (eA5)/Fc stripes (c,d) with or without bath treatment of ephrin-A5 (b) or netrin-1 (d). Middle panels: inverted images of EphA4 signals shown at left panels. Right panels: superimposed images of five explants from each experimental group representing the distribution of lateral LMC neurites. Quantification of lateral LMC neurites on first (pink) and second (pale) stripes expressed as a percentage of total EphA4 signals. Noted that no preference is detected when lateral LMC neurites are challenged with stripes of low levels of netrin-1 (10 ng/mL) or ephrin-A5 (50 ng/mL). Minimal number of neurites: 81. Minimal number of explants: 12. N, netrin-1; eA5: ephrin-A5; error bars = SD; ***P < 0.001; statistical significance computed using Mann-Whitney U test.