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. 2019 Aug 19;9:12009. doi: 10.1038/s41598-019-48519-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The ephrin-A5 induced increase in Neogenin protein levels in LMC growth cones may occur independently of protein synthesis and proteolytic degradation. (a) Quantification of Neogenin IF in LMC growth cones pre-treated for 20′ with either DMSO or CHX 50 µM followed by a 5′ treatment with either Fc or ephrin-A5 500 ng/mL. The inclusion of CHX did not significantly change Neogenin IF in growth cones treated with ephrin-A5 (p = 0.4177). (b) Relative to DMSO, a 20′ treatment with a protease inhibitor cocktail (P1860, 1/200) failed to increase the levels of F-actin (p = 0.0828), EphA4 (p = 0.0647) and Neogenin IF (p = 0.0769) in growth cones of LMC explant cultures. Data are shown as mean ± SEM, statistical significance was tested using a two-tailed unpaired sample t-test. (a,b) N = 4.