Table 1.

Functional comparison of endogenous IP3R levels to those in stable-rescued cells

		IP3R1	IP3R2	IP3R3
Stable rescue	Pool size (%)	63 ± 1	82 ± 1	69 ± 3
	EC50 (nM)	213 ± 44	146 ± 8	1664 ± 492
DKO	Pool size (%)	71	74	34
	EC50 (nM)	331	209	2895

IP₃ sensitivity was measured in permeabilized cells with cuvette-based fluorimeter. IP₃ sensitive pool size was determined as the percentage of the maximum Ca²⁺ signal measured at saturating IP₃ concentration relative to that at Tg addition (mean ± SEM, N = 37, 28, and 15 runs, respectively, from six independent experiments). EC50 values were determined by fitting sigmoidal, Hill, 3 parameter function on data points of dose-response measurements (N = 74, 55, and 60 points, respectively) For more details, see Supplementary Fig. 1C, D. DKO results are from¹⁹. Source data are provided as a Source Data file