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. 2019 Aug 19;5:130. doi: 10.1038/s41420-019-0210-6

Fig. 5. Lysosomal activity is essential for calcium-mediated cell protection against MPP+ toxicity.

Fig. 5

a MN9D cells were treated with 50 μM MPP+ in the presence or absence of 30 μM BAPTA-AM for 24 h. Cell lysates were subjected to immunoblot analyses using the indicated antibodies. Representative blots are provided. MN9D cells were treated with 50 μM MPP+ in the presence or absence of 30 μM BAPTA-AM plus b, c 750 nM rapamycin or d, e 50 μM CQ for 30 h. (b) LC3-II levels were detected by immunoblotting with anti-LC3B antibodies. c, d MTT reduction assays were performed to assess cell viability expressed as a percentage of the untreated control cells (100%). Bars represent the mean ± SEM of three independent experiments in triplicate. *p < 0.05; ***p < 0.001; NS, not significant. e After treatment with the indicated combination of drugs, MN9D cells were stained with 0.75 μM MitoTracker Red CMXRos and imaged using fluorescence microscopy. The scale bar represents 200 μm