Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 19;9:12076. doi: 10.1038/s41598-019-48369-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Determination of relative cell viability after electrical stimulation. Relative cell viability of electrically stimulated ASC for 72 hours versus unstimulated controls (CTRL set at 100%). Starting with a protocol of 4 V/cm, 6 ms pulses and 2 Hz, one parameter was changed per experiment while the other two parameters were kept at the original level. (A) Voltage was increased and decreased twofold. 4 V/cm had no effect on cell viability, whereas 2 V/cm increased viability and 8 V/cm decreased viability. (B) Pulse duration was set at 6 ms, 3 ms and 12 ms. Where 6 and 3 ms had no effect on cell viability, 12 ms decreased cell viability. (C) Only frequencies of more than tenfold the starting value showed a decrease in viability. Values are expressed as the mean percentage of relative viability (of ES vs. CTRL) + SD. n = 3 per parameter.