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. 2019 Aug 19;9:12076. doi: 10.1038/s41598-019-48369-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of the identified proteins with ELISA. (A) Concentrations of VEGF in the CM over time, ES vs. CTRL. The concentration at 72 h was significantly different for ES compared to CTRL (p = 0.04). The gradual rise in concentration visible in the time curve was representative for that of the other proteins. (B) MCP-1 was significantly increased in ES vs CTRL (p = 0.04), whereas the increase in PTX-3 did not reach statistical significance (p = 0.08). (C) Serpin-E1 was significantly decreased in ES vs CTRL (p = 0.03), whereas IGFBP-3, IL-8 and Serpin-F1 were not statistically different. (D) TSP-1 and TIMP-1 were both significantly increased in ES vs. CTRL (p = 0.03 and p = 0.04, respectively). Values displayed are mean + SD. *p < 0.05. n = 4 per group.