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. 2019 Aug 13;7:157. doi: 10.3389/fcell.2019.00157

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Copyright © 2019 Fisher, Thomas-Oates, Wood and Ungar.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Total enzymatic rate changes to maximize relative abundance of target glycans. Percentage changes in total effective enzymatic rates following fitting to maximize the abundance of bi-Sia₁ (blue), bi-Sia₂ (red), and tri-Sia₁ (green) fitted with variable enzyme localization (solid) and fixed enzyme localization (striped) relative to the parameters predicted for the WT HEK293T cell profile. Total effective enzymatic rates obtained from the different fits were adjusted to ensure that the transit time in each case matched that of the WT HEK293T simulation.