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. 2019 Aug 13;9:289. doi: 10.3389/fcimb.2019.00289

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Copyright © 2019 Lopes, Stylianou, Backman, Holmberg, Ekoff, Nilsson and Urban.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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C. neoformans infection increase MC survival. (A) Infection with capsular C. neoformans increased HMC-1 survival. Infections were performed using MOI of 5. Data from n = 6 (3). (B) Infection with capsular C. neoformans (MOI 5) increased viability of primary MCs. Data from n = 4 (3). (C) Secreted components from C. neoformans overnight cultures induced MC survival. Plotted is the delayed death of resting MCs incubated with increasing concentrations of supernatants of an overnight culture of C. neoformans plotted as fold change in relation to untreated MCs (dashed line). For all analyses significance was analyzed by multi-comparison using two-way ANOVA with Bonferroni post-test. ^*P ≤ 0.05; ^**P ≤ 0.01; ^***P ≤ 0.001; ^****P ≤ 0.0001; ns, not significant.