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. 2019 Aug 13;7:165. doi: 10.3389/fcell.2019.00165

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Copyright © 2019 EL-Deeb, EL-Adawi, EL-wahab, Haddad, EL Enshasy, He and Davis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Viability assay of the Mu extract with its fractions on PBMC and NK cells. (A) The viability assay test of the semi-purified mushroom fractions 1, 2, and 3 (Mu1, Mu2, and Mu3) with its crude extract on PBMCs using MTS Cell Proliferation Assay. All three Fractions showed abelites to induce cellular proliferations up to 120%. Data are mean and SD, ^****p < 0.0002, ^∗∗∗p < 0.001, ^∗∗p < 0.01, ^*p < 0.0370 and NS p = 0.423 with two way ANOVA with Turkey’s test, (B) The viability assay test of the semi-purified mushroom fractions 1, 2, and 3 (Mu1, Mu2, and Mu3) with its crude extract on PBMCs using MTS Cell Proliferation Assay, NK cells proliferations increased by using Mu1 and Mu2. Data are mean and SD, ^****p < 0.0001, ^*p < 0.08 and NS p = 0.6025 with two-way ANOVA with Turkey’s test.