TABLE 3.
Gene point mutation and amino acid changes in GyrA, GyrB, ParC, and ParE in the Mh clinical isolates.
| Isolate number | Antibiotic resistance MIC (μg/ml) | Mutation positiona | ||||||
| Nemonoxacin | Moxifloxacin | Levofloxacin | Ciprofloxacin | GyrA | GyrB | ParC | ParE | |
| Mh1 | 2 | 0.06 | 4 | 2 | − | − | Ser91Ile (A207G, G272T, 285G) | − |
| Mh2 | 4 | 2 | 8 | 4 | Ser153Leu (C458T, T513A, C528T, T606C) | − | Ser91Ile (G272T, A285G, C369T) | − |
| Mh3 | 8 | 8 | 16 | >16 | Ser153Leu (C458T, T513A, C528T, T606C) | − | Ser91Ile (G272T, A431G) | − |
| Mh4 | 8 | 8 | 8 | >16 | Ser153Leu (G408A, C458T, C528T, C594T, C660T) | − | Ser91Ile (G272T, T261A, A288G, C369T, C375T, C489G, G504A, T549C) | − |
| Mh5 | 2 | 0.125 | 4 | 2 | − | − | Ser91Ile (G272T, A207G, A285G, C369T, C375T) | − |
| Mh6 | 4 | 0.125 | 8 | 4 | − | − | Ser91Ile (G272T, T261A, A288G, C369T, C375T, C489G, G504A, T549C) | − |
| Mh7 | 2 | 0.125 | 8 | 4 | − | − | Ser91Ile (G272T, T261A, A288G, C369T, C375T, C489G, T549C) | − |
| Mh8 | 2 | 0.06 | 4 | 2 | − | − | Ser91Ile (G272T, A207G, A285G, A431G) | − |
| Mh9 | 8 | 8 | 8 | 16 | Ser153Leu (C458T, C528T, C594T, C660T) | − | Ser91Ile (G272T, A431G) | − |
| Mh10 | 4 | 8 | 8 | 8 | Ser153Leu (T363C, T369C, G408A, C458T, C528T, C594T, C660T) | − | Ser91Ile (G272T, A207G, A288G, A431G, T549C) | − |
| Mh11 | 1 | 2 | 4 | 8 | Ser153Leu (G408A, C458T, T606C, G627A) | − | Ser91Ile (G272T, T261A, A288G, C369T, C375T, C489G, T549C) | − |
| Mh12 | 2 | 4 | 8 | 16 | − | − | Ser91Ile (G272T, A431G) | − |
| Mh13 | 4 | 4 | 4 | 8 | Ser153Leu (G408A, C458T, T606C, G627A) | − | Ser91Ile (G272T, T261A, A288G, C369T, C375T, C489G, T549C) | − |
| Mh14 | 2 | 4 | 4 | 8 | Ser153Leu (A396G, G408A, A432G, C458T, C528T, A540T, C555T, C660T, C684T) | − | Ser91Ile (A207G, G272T, A285G, C369T, C375T) | − |
| Mh15 | 2 | 4 | 4 | 16 | Ser153Leu (C458T, T513A, C527T, T606C) | − | Ser91Ile (G272T, A431G, G460A) | − |
| Mh16 | 1 | 0.5 | 2 | 8 | Ser153Leu (T387C, A432G, T450C, C458T, C528T, C549T, A552T, C555T, T564A, C684T) | − | Ser91Ile (A207G, G272T, A285G, C369T, C375T) | Pro445Ser (C1333T) |
| Mh17 | 0.5 | 0.25 | 0.25 | 1 | − | − | − | − |
| Mh18 | 0.25 | 0.06 | 0.25 | 0.5 | − | − | − | − |
| Mh19 | 0.25 | 0.125 | 0.25 | 1 | − | − | − | − |
| Mh20 | 0.25 | 0.125 | 0.5 | 1 | − | − | − | − |
aThe information presented in parenthesis are mutations of the corresponding genes. Bold fonts are in correspondence with amino acid substitutions particularly. Others are synonymous mutations.