FIGURE 5.

EFhd2 affects tau liquid–liquid phase separation. (A) Wild type human EFhd2 (hEFhd2^WT) recombinant protein (4 μM) and equimolar concentration of recombinant tau (4 μM) were subjected to liquid–liquid phase separation in absence (–PEG; not shown) or presence (+PEG) of 10% Polyethylene glycol (PEG) as crowding agent plus or minus 10 mM CaCl₂- The images are representative of, at least, three independent experiments. Scale bar = 10 μm. (B) Co-incubated EFhd2 wild type protein (4 μM) and tau (4 μM) in the presence or absence of 10 mM CaCl₂ was subjected to FRAP. The graph shows fluorescence recovery with time in seconds (sec) after photo-bleaching (PB). The intensity was normalized to the first time point before photo-bleaching and shown as the mean and standard error of the mean (SEM) through time, n = 11.