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EFhd2 coiled-coil domain is required for affecting Tau liquid phase condensation, human EFhd2 coiled-coil deletion mutant (hEFhd2ΔCC) protein (4 μM) and equimolar concentration of recombinant Tau (4 μM) were subjected to liquid–liquid phase separation in presence (+PEG) of 10% Polyethylene glycol (PEG) as crowding agent plus or minus 10 mM CaCl2- The images are representative of, at least, three independent experiments. Scale bar = 10 μm.
