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. 2019 Aug 8;178(4):901–918.e16. doi: 10.1016/j.cell.2019.07.029

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© 2019 Medical Research Council on behalf of UKRI and Imperial College London

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Figure S5 — Enterocyte-Specific Knockdown Screen to Identify the Signaling Pathway Driving Intestinal Sex Differences in Sugar Gene Expression, Related to Figure 4

(A) RT-qPCR expression analysis of midgut-specific sugar genes (indicated at the bottom of the graph) in male (M) and female (F) flies following enterocyte-specific knockdown of signal transduction components. The specific genes targeted from top to bottom are: Allatostatin A receptor 2 (Asta-R2), Tachykinin-like receptor at 99D (TkR99D), rickets (rk), punt (put), Insulin-like receptor (InR), LDL receptor protein 1 (LRP1), baboon (babo), Lipophorin receptor 1 (LpR1), Megalin (mgl), Lipophorin receptor 2 (LpR2), torso (tor), Ion transport peptide (ITP), Toll (Tl), bigmax (Mlx), Methoprene-tolerant (Met), Niemann-Pick type C-2e (Npc2e), Neural Lazarillo (Nlaz), grindelwald (grnd), slimfast (slif), Adiponectin receptor (AdipoR), Ecdysone receptor (EcR), ultraspiracle (usp), breathless (btl), germ cell-expressed bHLH-PAS (gce).

(B–D) RT-qPCR expression analysis of the same midgut-specific sugar genes in male (M) and female (F) flies with CCHamide-2 receptor (B, CCHa2-R^TAL/KO), Adipokinetic hormone (C, Akh^AP/A), and Adipokinetic hormone receptor (D, AkhR^1/Δ) null mutations. None of these genetic manipulations affected the sexual dimorphism in intestinal sugar gene expression.

In all panels, n denotes the number of group of flies analyzed for each genotype (each group contains 20 flies). Asterisks highlighting significant comparisons across sexes are displayed in gray boxes at the bottom of the graphs. See Table S4 for a list of full genotypes.