Table 1.
Plasmids and strains used in this study.
| Plasmid or strain | Descriptiona,b | Source |
|---|---|---|
| PLASMID | ||
| pGEM-T Easy | TA cloning vector; Ampr | Promega |
| pUAMS4 | pGEM-T Easy::PflgB-aacC1 (AscI-flanked); Gentr, Ampr | This study |
| pUAMS169 | pGEM-T Easy::aacC1 (Flanked with 5′NdeI and 3′AscI); Gentr, Ampr | This study |
| pUAMS177A | btpA::PflgB-aacC1 mutagenesis construct; Gentr, Ampr | This study |
| pUAMS238 | btpA::aacC1 mutagenesis construct; Gentr, Ampr | This study |
| STRAIN | ||
| B. turicatae 91E135 (Oz1) | B. turicatae tick isolate | Taylor et al., 1991; Schwan et al., 2005 |
| B. burgdorferi 297 | B. burgdorferi human isolate | Hughes et al., 1992 |
| E. coli TOP10F' | F′ [lacIqTn10(Tetr)] mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 nupG ΔlacX74 recA1 araΔ139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 | Life Technologies |
| B. turicatae btpA::PflgB-aacC1 | btpA mutant with gentamicin resistance marker under transcriptional control of the flgB promoter | This study |
| B. turicatae btpA::aacC1 | btpA mutant with promoterless gentamicin resistance marker | This study |
a
Amp, ampicillin.
b
Gent, gentamicin.