Figure 1.

Cell-free expression of the reporter protein deGFP in the all-E. coli TXTL system using the plasmid P70a-deGFP. (a) Kinetics of deGFP synthesis at 5 nM plasmid showing three regimes: (I) transient regime, (II) steady state, (III) plateau (gene expression stops). (b) Maximum rate of deGFP synthesis as a function of the plasmid concentration. Two regimes are observed: linear (L) at low plasmid concentration, saturated (S) at high plasmid concentration. (c) Schematic of the model showing most of the components included in the model. (d) Final equation set of the model. Equations 20 and 21 have to be solved for E₀ and R₀ respectively.