Fig. 4.

RBOHD transcript and protein abundance in delt1-2 mutants

(a) Diagram of primer position used in (b). (b, c) RBOHD transcript levels. Total RNA was prepared from leaves of 7-d-old plants. RBOHD transcript levels were determined by semi-quantitative (b) and quantitative (c) RT-PCR. EF-1α was used as the reference gene. Values are mean±standard error (SE) of three biological repeats in (c). (d, e) RBOHD protein abundance. Total (d) and plasma membrane (e) proteins were extracted and detected by immunoblot analysis with anti-RBOHD antibody. The rbohD T-DNA null allele was used to verify the specificity of anti-RBOHD antibody. Rubisco (RBCL) and chitin elicitor receptor kinase 1 (CERK1) were used as loading controls. The experiment was carried out twice with similar results