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. 2019 Aug 13;12:193. doi: 10.3389/fnmol.2019.00193

Figure 4.

Figure 4

TIGAR reduced high glucose-induced apoptosis through regulating autophagy in Neuro-2a cells. (A–D) Autophagy inhibitors 3-MA and chloroquine diphosphate (CQ) abolished the effect of TIGAR on neuronal apoptosis in high glucose conditions. Western blot analysis of Bcl-2, Bax, active caspase-3 and total Caspase-3 under high glucose condition in each group (n = 5 per group). (E) Immunofluorescent staining of active Caspase-3 and quantification of active Caspase-3-positive cells in each group (n = 9 per group). Scale bar = 40 μm. *p < 0.05, **p < 0.01 vs. the control group, #p <0.05, ##p < 0.01 vs. the HG group, &&p < 0.01 vs. the HG+TIGAR group, two-way ANOVA.