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. 2019 Aug 13;12:193. doi: 10.3389/fnmol.2019.00193

Figure 5.

Figure 5

Overexpression of TIGAR reduced STZ-induced autophagy impairment and memory loss in mice. (A) Western blot analysis of NOS1, LC3B, Beclin-1, TIGAR and P62 after intraperitoneal injection of STZ and microinjection of Lenti-TIGAR in the hippocampus of mice (n = 5 per group). (B) Western blot analysis of Bcl-2 and Bax in the hippocampus of each group (n = 5 per group). (C) Locomotor activity was quantified 4 weeks after microinjection of Lenti-GFP or Lenti-TIGAR into the hippocampus of mice (n = 7 per group). (D) Schematic representation of the location-dependent object recognition memory (ORM) test (Left). Quantification of discrimination index in each group (n = 7 per group; Right). (E) Schematic representation of the novel ORM test (Left). Quantification of discrimination index in each group (n = 7 per group; Right). *p < 0.05, **p < 0.01 vs. the control group, #p < 0.05, ##p < 0.01 vs. the STZ group, one-way ANOVA.