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. 2019 Aug 13;10:1926. doi: 10.3389/fimmu.2019.01926

Figure 1.

Figure 1

The degree of liver fibrosis in chronic HCV infection is associated with altered circulating CD8+ T-cell subset distribution. CD8+ T-cells were isolated from PBMCs for analysis of subset distribution. The proportions of cell CD8+ T-cell subsets were distinguished based on surface marker expression by flow cytometry as follows: Naïve (CD45RA+CCR7+CD27+/−), Effector (E, CD45RA−CCR7−CD27−), Early Effector Memory (e-EM, CD45RA−CCR7−CD27+), Late Effector Memory (l-EM, CD45RA+/−CCR7−CD27−), and Central Memory (CM, CD45RA−CCR7+CD27+/−). (A) Representative dot plots of the lymphocyte gate (forward vs. side scatter) and phenotype strategy are shown. The distribution of (B) uninfected controls (n = 9) and treatment naïve HCV+ individuals with (C) minimal (Metavir score F0-1, liver thickness ≤7.0 kPa, n = 9) or (D) advanced liver fibrosis/cirrhosis (F4, ≥12.5 kPa, n = 5) are shown with pie-charts [mean percentages (%), subset S.D. < ±10%]. Representative dot plots of CD8+ T-cell subset distribution in the (E) HCV+ (F0-1) and (F) HCV+ (F4) study groups are shown.