TABLE 1.
Opine and molecular characterization of crown gall samples collected from Hungary in 2014.
| Sample ID | Cultivar |
Opine assay |
PCR assay |
|||
| Phenantrene-quinone | Silver-nitrate | Reversed silver-nitrate | PGF-PGR | VirD2A-VirD2E | ||
| H14_1 | Lilla | Octopine | – | – | + | – |
| H14_2 | Lilla | – | – | – | – | – |
| H14_3 | Lilla | Octopine | – | – | – | – |
| H14_4 | Lilla | Octopine | – | – | – | – |
| H14_5 | Lilla | – | – | – | – | – |
| H14_6 | Lilla | Octopine | – | – | – | – |
| H14_7 | Medina | – | – | Vitopine | + | – |
| H14_8 | Medina | – | – | Vitopine | – | – |
| H14_9 | Medina | Octopine | – | – | – | – |
| H14_10 | Teréz | – | – | – | – | – |
| H14_11 | Teréz | Octopine | – | – | + | – |
| H14_12 | Teréz | – | – | – | – | – |
| H14_13 | Teréz | – | – | – | – | – |
| H14_14 | Teréz | – | – | – | – | – |
| H14_15 | Teréz | Octopine | – | – | – | – |
| H14_16 | Teréz | Octopine | – | – | – | – |
Presence of opine in the opine assay is indicated by the name of opine type detected by the specific test while minus signs indicate the absence of opine. Plus and minus signs in the PCR assay column indicate the presence and absence, respectively, of PCR products corresponding to the pehA gene of Allorhizobium vitis (PGF–PGR) or virD gene of A. tumefaciens (VirD2A–VirD2E) in the extracted crown gall DNA.