Figure 1.

Heterogenous distribution of muscarinic and purinergic receptors. (a) Fluorescence localisation of purinergic P2Y2 receptors and muscarinic M3 receptors in the endothelium. Representative images (from left) show the endothelial cell boundaries as revealed by PECAM-1 labelling (green; anti-CD31/PECAM-1), P2Y2 receptor (red; anti-P2Y2) distribution, M3 receptor distribution (blue; fluorescent M3 receptor antagonist, 100 nM) and overlay of all three. The receptors distribution was not uniform across the endothelium and there was relatively little overlap of purinergic and muscarinic receptor staining. (b) Expanded view of the endothelial images shown in (a). The expanded region is shown by the red box in (a) left-panel. All scale bars = 50 μm. Modified from Ref. [50^•] with permission.