Figure 4.

Interaction of AdipoR1 confers migration and invasion inhibitory effect of APN in RCC cells.

(A) Migration of AdipoR1-knockdown and control cells was evaluated by Transwell assay after treatment with APN (10 μg/m) for 72 h.

(B) Graphs show the relative number of migratory cells (n = 4).

(C) Invasion of AdipoR1-knockdown and control cells were evaluated by Transwell assay after treatment with APN (10 μg/ml) for 72 h.

(D) Graphs show the relative number of invasive cells (n = 4).

(E) Invasion and migration of AdipoR1-overexpression, AdipoR2-overexpression and control ACHN cells were evaluated by Transwell assay after treatment with or without 10 μg/ml APN for 72 h.

(F) Graphs show the relative number of migratory and invasive cells (n = 4).

Results are presented as mean ± SD. *P < .05, **P < .001 compared with untreated cells.