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. 2019 Aug 19;158(1):35–46. doi: 10.1111/imm.13093

Figure 1.

Figure 1

Flow cytometry analysis of isolated mouse colonic intraepithelial lymphocytes (IEL) subsets during dextran sulphate sodium (DSS)‐induced colitis. (a) % CD3+ cells of isolated colon IELs. (b) CD3+ cells were gated, and TCRγδ expression was measured. (c) TCRγδ+ cells were gated, and CD8αα expression was measured. (d) CD3+ cells were gated, and TCRαβ expression was measured. (e) TCRβ+ cells were gated, and CD4 expression was measured. (f) TCRβ+ cells were gated, and CD8αβ expression was measured. (g) TCRβ+ cells were gated, and CD8αα expression was measured. Each circle represents an individual mouse (n = 10), and the mean is shown. Statistical analysis was performed using Student's t‐test, *P < 0·05, **P < 0·01, ***P < 0·001.