Flow cytometry analysis of programmed cell death protein‐1 (PD1), T‐cell immunoreceptor with Ig and ITIM domains (TIGIT) and Herpes virus entry mediator (HVEM) expression in different intraepithelial lymphocyte (IEL) subsets from mouse colons during dextran sulphate sodium (DSS)‐induced colitis. (a) PD1, TIGIT and HVEM expression in TCRγδ+ T‐cells, (b) CD8αα TCRγδ+ T‐cells, (c) CD4 TCRαβ+ T‐cells, (d) CD8αβ TCRαβ+ T‐cells and (e) CD8αα TCRαβ+ T‐cells. Each symbol represents the mean of 10 mice. Statistical analysis was performed using Student's t‐test, *P < 0·05, **P < 0·01, ***P < 0·001.