Figure 3.

Generation of GGCT Knockout Mouse Model and GGCT^−/− Mice Are Viable

(A) ES cell-targeting strategy for generating GGCT conditional knockout mice.

(B) PCR genotyping results for GGCT conditional knockout founder mice and GGCT^−/− mice.

(C) Western blot analysis of GGCT^+/+ and GGCT^−/− MEFs with anti-GGCT antibody; β-actin served as loading control.

(D) Genotyping results of GGCT^+/− mouse mated with GGCT^+/− mouse and GGCT^+/− mouse mated with GGCT^−/− mouse. In both cases, the genotype distributions of offspring are in line with Mendel's law, implicating that GGCT deletion is compatible with normal mouse embryonic development.

(E) Representative pictures of two-months-old male and female GGCT^+/+ and GGCT^−/− mice.