Figure 4.

GGCT^−/− MEFs Show Proliferation Inhibition, Senescence, and Resistance to Automatic Transformation Phenotypes

(A) Cell proliferation assay. 10 × 10⁴ MEFs of indicated genotypes are seeded into 3.5-cm tissue culture dishes, and the cell numbers are counted every 3 days. Error bars represent mean ± SD of three experiments.

(B) Phase contrast images of primary and large T-transformed wild-type and GGCT^−/− MEFs. Nearly all late-passage (P7) GGCT^−/− MEFs are senescent, whereas same-passage primary wild-type or transformed wild-type, GGCT ^−/− MEFs do not show significant senescent features. White arrows indicate typical senescent cells, which show flat morphology and double nuclei phenotypes. Scale bar, 50 mm.