Figure 4.

Functional Analysis of iPSC-CMs Using MEA Following I_Ks Blockade

(A) Representative traces of FP in Control- and LQT1^A344Aspl-iPSC-CMs.

(B) FPDc at baseline was longer in LQT1^A344Aspl-iPSC-CMs than in Control-iPSC-CMs (independent experiments, n = 45 and 21 from independent differentiation experiments, n = 16 and 6 in Control- and LQT1^A344Aspl-iPSC-CMs, respectively; mean ± SEM; p < 0.05; unpaired Student's t test). ^∗p < 0.05.

(C) Representative traces of the FPD following administration of 10 μmol/L (red), 50 μmol/L (green), and 100 μmol/L (blue) chromanol 293B in Control- and LQT1^A344Aspl-iPSC-CMs.

(D) Averaged %ΔFPDc in each cell line. %ΔFPDc upon treatment with 100 μmol/L chromanol 293B was significantly smaller in LQT1^A344Aspl-iPSC-CMs than in Control-, LQT2^A422T-, and LQT3^N406K-iPSC-CMs (independent experiments, n = 6, 13, 6, and 9 from independent differentiation experiments, n = 3, 5, 3, and 3 in Control-, LQT1^A344Aspl-, LQT2^A422T-, and LQT3^N406K-iPSC-CMs, respectively; mean ± SEM; p = 0.001; two-way repeated measures ANOVA). ^∗p < 0.05; Fisher's LSD post hoc test.